This comprehensive masterclass is designed for nurses and healthcare professionals, focusing on the critical role of biotics in pediatric care. The course offers in-depth insights into the first 1000 days of life, emphasizing the importance of breastfeeding, addressing malnutrition, and understanding the intricate relationship between gut microbiota and overall health. Participants will explore the utilization of probiotics, prebiotics, and postbiotics, alongside the significance of human milk and optimal nutrition support in pediatric intensive care units (PICU).